Homogenization of human tissues via picosecond-infrared laser (PIRL) ablation

Analytics
Giving a closer view on the in-vivo composition of protein species as compared to mechanical homogenization Enzymatic protein modifications during tissue homogenization are responsible for changes of the in-vivo protein species composition. Cold vaporization of tissue by PIRL-DIVE is comparable with taking a snapshot at the time of the laser irradiation of the dynamic changes that occur continuously under...
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PIRL scalpel wound healing

Wound healing is a complex process which is affected by metabolism-specific and immunological interactions as well as by mechanical stimulations. At the end of the healing-process of the impaired tissue, a scar usually remains. Scar tissue is the main limitation in the most critical surgeries where one wants to maintain func­tion. After spinal surgeries or surgery of the vocal...
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The DIVE principle – the mechanism behind PIRL

Physics
Desorption by impulsive vibrational excitation (DIVE) represents a novel mechanism for laser ablation that is suitable for all different kinds of water-containing samples. During a typical laser ablation procedure, material is removed from a surface by focusing a pulsed laser beam on a target area, the result being evaporation or plasma formation and ejection. While the DIVE principle...
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PIRL DIVE-extraction of proteins

Analytics
Ultrafast extraction of proteins from tissues using desorption by impulsive vibrational excitation. The extraction of proteins from tissue samples represents a critical and time-consuming step in every proteomic and protein analytical approach. In general, protein extraction includes a number of different experimental steps like homogenization, lyzation and protein isolation varying in dependence of tissue type and proteins of interest....
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