Giving a closer view on the in-vivo composition of protein species as compared to mechanical homogenization

JProt_Kwiatkowski_SurePIRL_HPEnzymatic protein modifications during tissue homogenization are responsible for changes of the in-vivo protein species composition. Cold vaporization of tissue by PIRL-DIVE is comparable with taking a snapshot at the time of the laser irradiation of the dynamic changes that occur continuously under in-vivo conditions. At that time point all biomolecules are transferred into an aerosol, which is immediately frozen.

Published as open-access in the Journal of Proteomics, DOI: 10.1016/j.jprot.2015.12.029 [1].

Mass spectrometric data is available on the PRIDE repository [2] with the dataset identifier PXD003434.

References
  1. Kwiatkowski M, et al. Homogenization of human tissues via picosecond-infrared laser (PIRL) ablation: Giving a closer view on the in-vivo composition of protein species as compared to mechanical homogenization. J Proteomics. 2016 Jan 8.
  2. Vizcaíno JA, et al. The PRoteomics IDEntifications (PRIDE) database and associated tools: status in 2013. Nucleic Acids Res. 2013 Jan;41.
http://www.surepirl.eu/wp-content/uploads/2016/01/JProt_Kwiatkowski_SurePIRL_HP.pnghttp://www.surepirl.eu/wp-content/uploads/2016/01/JProt_Kwiatkowski_SurePIRL_HP-200x134.pngadminAnalytics
Giving a closer view on the in-vivo composition of protein species as compared to mechanical homogenization Enzymatic protein modifications during tissue homogenization are responsible for changes of the in-vivo protein species composition. Cold vaporization of tissue by PIRL-DIVE is comparable with taking a snapshot at the time of the laser...